![]() Triangle® credit cards are issued by Canadian Tire Bank. *The Triangle Rewards Program is owned and operated by Canadian Tire Corporation, Limited. Our Lowest Prices of the Year are defined as the period between January 1 to December 31, 2022. ✛ased on the promotion prices as advertised in accordance with this flyer's sale dates. Visit Online Ordering for more information. Bulk items will only be delivered within a 100km radius of the Store. Shipping fees and delivery times vary depending on location, size and weight of the item(s) and is only available within the province of the Canadian Tire retail location (“Store”) from which the item(s) was purchased. We reserve the right to limit quantities. Items may be display models or not exactly as shown and may not be available in all stores. ◊Pricing, selection, and availability of store clearance items are determined by each store. ®/TMSport Chek is a registered trademark of FGL Sports Ltd, used under licence. ![]() ®/TMMark’s is a registered trademark of Mark’s Work Warehouse Ltd, used under licence. Visit Triangle Rewards: Sign Up & Earn Today for more information. Bonus CT Money collected from online orders will be applied to the member’s Triangle Rewards™ account within 5 weeks of the purchase date. The offered rate is exclusive of any bonus or promotional offers or redemption transactions. Not all items sold at Canadian Tire earn CT Money. Any bonus multiplier is based on the base rate of collecting CT Money. You cannot collect paper Canadian Tire Money on bonus offers. † In the form of electronic Canadian Tire Money® (CT Money®). Examples of borrowing costs (rounded to the nearest cent) assuming that all charges are purchases bearing interest at the regular annual rate of 20.99%, a 30 day month, no charges made on special payment plans and no other fees, additional payments or other changes are: The Triangle Mastercard and the Triangle World Elite Mastercard do not have an annual fee. The billing period covered by each statement can be from 28-33 days. For residents of Quebec, the period between the statement date and the due date for payment is 26 days. The minimum payment is the sum of (a) the greater of: (i) interest and fees shown on your statement + $10 or (ii) 5% of the New Balance, excluding amounts on special payment plans, (b) any balance over your credit limit, (c) any amounts past due not included in (b) above, and (d) the amount of any equal payments plan instalments then due. Some applicants may receive a higher or lower regular annual rate depending on a credit evaluation. Offer subject to change without notice.Īdditional information for residents of Quebec only: The regular annual rate for persons applying for the Triangle Mastercard and the Triangle World Elite Mastercard is 22.99% for cash transactions and related fees and 20.99% for all other charges. Any unpaid portion not received by the due date will no longer form part of the equal payments plan and interest will accrue on that amount from the day after the date of your next statement at the applicable regular annual rate. Each month during an equal payments plan you are required to pay in full by the due date that month’s equal payments plan instalment. ![]() There is no administration fee charged for entering into a special payments plan. However, if we do not receive the full minimum due on a statement within 59 days of the date of that statement, or any event of default (other than a payment default) occurs under your Cardmember Agreement, all special payment plans on your account will terminate and (i) you will then be charged interest on the balances outstanding on such plans at the applicable regular annual rate from the day after the date of your next statement, and (ii) the balances outstanding will form part of the balance due on that statement. ![]() Interest does not accrue during the period of the plan. Dealers may sell for less.įinancing available is “Equal payments, no interest” for 24 months (unless otherwise stated) and is only available on request, on approved credit and on purchases of $150 (unless otherwise stated) or more (Gift Cards excluded) made with your Triangle credit card at Canadian Tire, Sport Chek, Mark’s, L’Équipeur, Atmosphere, Sports Rousseau, Hockey Experts, L’Entrepôt du Hockey and participating Sports Experts. **Online prices and sale effective dates may differ from those in-store and may vary by region. ![]() ±Was price reflects the last national regular price this product was sold for. The tire producer / manufacturer and Canadian Tire uses this fee to pay for the collection, transportation, and processing of used tires.ĬANADIAN TIRE® and the CANADIAN TIRE T riangle Design are registered trade-marks of Canadian Tire Corporation, Limited. △The tire producer / manufacturer of the tires you are buying, and Canadian Tire is responsible for the recycling fee that is included in your invoice. ![]()
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![]() Fabrication and installation can also be more labor-intensive, which may add to the final cost. Waterfall countertops add significance to your kitchen by creating a visually appealing focal point. As with any concrete countertop, custom elements or special finishes will significantly increase the overall price tag. More material will be required, but the cost per square foot is typically the same. The cost of a waterfall countertop is generally no different than what you’d pay for a conventional concrete countertop. In addition to being lighter in weight than conventional concrete, GFRC is easily molded and high in flexural strength, making it ideally suited for three-dimensional concrete creations like waterfall countertops and furniture. It’s also possible to add waterfall edges to an existing concrete countertop or kitchen island if there is enough overhang to accommodate new pieces of the same thickness.īecause waterfall edges significantly increase the overall weight of the countertop, they are often made using glass-fiber-reinforced concrete. The end pieces can be cast separately from the countertop itself, the countertop and ends can be cast as one piece (which eliminates seam lines), or the countertop and side panels can be constructed of plywood or particle board and then covered with a concrete microtopping. There are a number of ways to build a waterfall concrete countertop. How Do You Build a Waterfall Concrete Countertop? Ideas for Concrete Waterfall Countertops & IslandsĬheck out these custom projects to see how concrete waterfall countertops can enhance your kitchen:Ī waterfall-style concrete grill station with stainless steel appliances provides ample workspace for outdoor cooking. In addition to countertops, waterfall edges can also be used for concrete bathroom vanities, kitchen islands, bar tops, and furniture. ![]() What’s more, a waterfall edge puts more of your beautiful concrete on display, allowing you to appreciate it from a different perspective.įind concrete countertop contractors near me.Īlthough the waterfall style is not new (it became popular during the Art Deco era of the 1930s) the strong, clean lines make it ideally suited for contemporary design schemes. This trendy design option is not only big on wow factor, it can serve many practical purposes as well, such as providing a framework for built-in cabinetry, hiding appliances, or concealing plumbing. ![]() One of the unique looks you can achieve with concrete countertops is a waterfall effect, where one or both ends of the countertop drop straight down to the floor, like water going over a ledge. Design Ideas for Concrete Waterfall Countertops See how to transform a traditional concrete countertop into a modern showpiece with this sophisticated design option. ![]() ![]() More details and images are available in this blog post. There are also improvements to message reactions, custom notification sounds, and the promise that "all browsers" will be supported. Other new features to look forward to include the Skype Universal Translator which offers real-time translations of conversations. Now if you want to show off your pet's sleeping habits or need to tutor someone while showing multiple angles, it's as easy as grabbing your mobile phone or whatever device you have handy and scanning a QR code to add it to the call as another camera! We match you with your video stream and you can impress everyone on the call. Have you ever wanted to show off your pet to a friend while on a video call on your computer, but you couldn't get your dog to sit still or your camera to move quickly enough to show them? We've made it way easier and more fun to do that with a new feature we call "TwinCam". On the subject of being able to use more than one camera at once, Microsoft says: Microsoft is also making it possible to hide participants that are taking part in a call using audio only, as well as giving you the option of disabling video feeds if bandwidth issues necessitate this. Part of the redesign sees the layout more closely resembling that found in Teams, with the idea being that visibility of participants is improved through the inclusion of larger video feed windows. The redesign of the app is the most obvious change that is coming to Skype, including a completely revamped call stage. How to upgrade to Windows 11 for free right now.How to sign into Windows 11 automatically.Most people neither know nor care about Windows 11.Store the image in a central location that everyone in your business can access, such as a network share, shared OneDrive for Business folder, Dropbox, etc. In addition to a makeover, in the pipeline is a raft of new features including call recordings, animated backgrounds and support for multiple cameras across multiple devices with TwinCam. Tips for creating a logo for meeting invitations Create an image that is no more than 188 pixels wide by 30 pixels tall (its quite small). ¡Es el logo de Skype que reduce o quizás anula nuestras facturas de teléfono A partir de ahora, es posible llamar a quien quieras, esté donde esté. ![]() ![]() Promising that it will be "fast, playful, delightful and buttery smooth", Microsoft has revealed some of what it has in store for Skype. ![]() But this does not mean that Microsoft has given up on this particular messaging tool, and the company is keen to let users know that they have not been forgotten. The last icon show what your current state (Formal active. In a world of Zoom, Google Meet, Slack, Microsoft Teams et al, Skype has somewhat fallen out of favor. Some controls will only be active when you are a supervisor. ![]() ![]() Some also allow parallel recording, writing two tapes at once. This means that you can switch between tapes for playback, but it also makes it very easy to copy content from one tape to another. A dual cassette deck has slots for two tapes. Different decks can play different numbers of tapes.This is useful if you want to record slow motion audio, alter the tone of your music, or just enjoy some fun effects. ![]() This lets you slightly alter the tape speed, which also alters the pitch of the audio.
![]() ![]() On September 14, a documentary was released about the event for sponsors on their website. Their first event was held on February 20, 2015, at the Moody Theater in Austin, Texas. Let's Play Live! is an on-stage event produced by Rooster Teeth in which the Achievement Hunter members play video games and comedy sketches in front of a live audience. ![]() įrom 2015, Achievement Hunter would feature celebrities as guest collaborators in some of their Let's Play videos such as Rahul Kohli, Laura Bailey, Travis Willingham, Dante Basco and pop punk band Neck Deep. Jones serves as the main host of the show and makes the most frequent appearances alongside other Achievement Hunter employees and special guests. The first official episode aired to the public after the initial tests. Four test episodes aired live and were only available to "Sponsors" on their website. A weekly podcast titled Off Topic was announced in early October by Michael Jones. ĭooley was promoted to a main host in October 2015, during an episode of Let's Play Minecraft. Additionally, they moved out of Rooster Teeth's Stage 5, Austin Studios office into their own office nearby. In mid-2015, Achievement Hunter launched a new YouTube channel, named after themselves, thereon they upload series of videos that were previously uploaded to the Rooster Teeth channel. In April 2015, Narvaez departed from the company as a full-time employee to focus on Twitch livestreaming, although he continues to voice his character for the company's X-Ray and Vav animated series. In early 2015, Achievement Hunter hosted the first Let's Play Live at the Moody Theater in Austin, Texas. At RTX in July 2014, community members Matt Bragg and Jeremy Dooley were hired as editors and secondary hosts at Achievement Hunter. This charity live stream has become an annual event, with 2014's Extra Life live stream raising over $442,000 for Children's Miracle Network. With appearances from other Achievement Hunter members and various Rooster Teeth employees, the live stream managed to raise $340,000. In October 2013, Pattillo hosted a 24-hour live stream benefiting Extra Life, a charity benefiting hospitalized children. ![]() Later in 2013, the company retired Achievement HORSE, replacing it with Achievement HUNT, in which a variety of employees challenge each other until the word "HUNT" has been spelled. In April 2013, Achievement Hunter launched their own channel on YouTube, which hosts a variety of their videos. In early 2013, Achievement Hunter introduced a competition series titled VS, in which the employees challenge each other to compete in games. Later, in September 2012, contract editor Franco Scarcello created Five Facts, which was generally hosted by Pattillo and Ramsey and focused on little-known facts about various video games it was not renewed for a sixth season in May 2016. was hired in April 2012, having worked as a contract host for multiple years. In March 2012, Achievement Hunter debuted Game Night, in which Ramsey and Caleb Denecour play games with community members the series switched to a live stream format in 2014. Īchievement Hunter hired Gavin Free in 2012, after he had assisted with the company for many years. Weekly Let's Play videos focusing on Grand Theft Auto games are also released. ![]() The success of the videos resulted in a weekly sub-series in 2012, in which the main hosts play Minecraft. In December 2011, Achievement Hunter also began creating Let's Play videos. Four new shows were introduced to Achievement Hunter in 2011 one of them, Rage Quit, led to the official hiring of Michael Jones as a full-time staff member in August 2011. In 2011, Achievement Hunter hired Ryan Haywood as an editor and manager Haywood later began being featured in videos as a host in March 2012, becoming one of the main crew members. Geoff Ramsey's interest in gaming achievements led to the creation of Achievement Hunter. ![]() ![]() Suddenly the warning light for AWD failure came on followed by the engine light and all other warning lights. In May 2022, an owner from Michigan was driving on the Interstate 50 highway in Iowa with cruise control set at 70 mph. The airbags also didn’t deploy in the incident, mentioned above, where a fire wiped out an RX 350. So, it stands to reason that if the driver side airbag hadn’t deployed, the injuries could have been worse. The front passenger side test gets a 4-star ranking and the front driver side only 3. Their rating for the 2022 Lexus RX 350 frontal crash tests aren’t great. The NHTSA undertakes 5-star safety ratings on most cars so that anyone searching for a car knows which are safer than others. “I suffered a concussion and a herniated disc.” On impact, both airbags deployed on the driver’s side (front and back), but not on the front passenger side. Both people in the car were wearing seatbelts at the time. The impact shifted the RX 350 and it was hit again on the front driver side of the vehicle. When they don’t work in an accident, people’s lives are at risk.Īn Alabama-based complaint from a passenger who was in an accident states that the vehicle was struck from behind while in standstill traffic. “I’m told transmission is ‘normal’, (the) mirror issue is known and has no fix, and not all of the squeaks/rattles were addressed.” Lexus RX Airbag ProblemsĪirbags are part of every vehicle’s safety equipment. Rating the value of this SUV 2 out of 5 stars, the reviewer goes on to share dealership response. The SUV rattles and squeaks in multiple places and the rearview mirror on the driver’s side vibrates, making it difficult to use. One review that suggests consumers “look elsewhere for (a) mid-size SUV” says the decision to buy a 2022 RX 350 was because of “Lexus’ reputation for superior quality and reliability.” But after driving the SUV for a week, the verdict is that the ride isn’t smooth, and the transmission is jerky and surges at lower speeds. ![]() ![]() In addition to complaints to the NHTSA, negative reviews posted on Edmunds talk about road noise, vibrations issues, and jerky transmission. These include malfunctioning airbags, explosive sunroofs, leaking fuel, and vibrating mirrors.Ī complaint filed as an “unknown or other” problem describes how fire destroyed an RX 350 on an interstate highway in 20 minutes. There are only five complaints on record as at mid-September 2022, but they highlight significant issues. Most Problems with the Lexus RX 350Īccording to NHTSA records, problems are varied. While the RX 350 is generally regarded as a reliable SUV, it’s far from perfect, as complaints to the National Highway Traffic Safety Administration (NHTSA) and other platforms reveal. There have been four generations of the RX, with the fifth scheduled for release as a 2023 model. The model name chosen to represent the bright new future for these cars at the start of the 21st century. ![]() ![]() 3rd episode opened (but unfinished) in the dating game.Sewer Shark expanded to 2 variants rather then 1 (plus events streamlined to reduce file size.lessons learned from Sim City).Sim City StinkHole adventure had to be rebuilt.mostly for the better.Locks out alternate sims (other than Grimm Invaders), Yin's quest, and Deeda's free shifting event.Teams RWBY, JNPR, SARA, CLEN, WILD, and LIMS can be started as a team.A vault full of cansiter-type androids can be found in Menagerie (requires Indra).Power System and A.I.s found in Vale-Mountain Glenn tunnel (lower right and upper right, respectively).Builder and/or her workshop found in Mountain Glenn.Not available to Sarah and Indra mains as their special shops replace it (actually, they were there first).15 minute time limit, or bet 2k on a 5 minute limit for a 10k reward.Jaune-Weiss and Ruby-Weiss are all that's currently available.Įscape Room Emporium (center-right of Vale).Many, many dates planned, but idea well dried up.Accessed by black car in Vale (back half, from bottom, facing up).OTP Gauntlet accessed in upper left of Menagerie.Character regions overseen by the S.S.Renora.accessed via a rowboat found in the upper left of the Patch-Vale bridge.5 regions based around what character they're fighting over, plus OTP Gauntlet.B-Side - Could probably be its own game.lots of dirty jokes.Red vs Blue (Blood Gulch) - Pick a side, capture the flag.Grimm Invaders - Sorta-kinda Space Invaders-ish.points traded for rewards, more for fast kills.Zombie Survival - Urban, Rural, Freeway, and Interstate.Puzzle Maze (Wonderland) freely accessed in each kingdom (rabbit hole in Vale, half open door in Menagerie, talking dog in Mistral).Īlternate Simulations/Mini-Games (all found in the Server Room under the Vale warehouse):.Horde Tower freely accessed in Menagerie (NOT low level content). ![]() Master access granted by Vale barkeeper after significant number of combat victories (150).Bad guys access granted by Torchwick (or generic substitute) in the abandoned warehouse in lower right of Vale after a modest number of combat victories (25 - makes Deeda unavailable if she isn't your main).Good guys access granted by Glynda in the upper right of Vale after small number of combat victories (15).Similar board in Menagerie for treasure hunts/points of interest.Boards in Mistral are all together in the upper right of the map.Boards in Vale are on some of the buildings.Roughly half the areas, a third of the initial tour, and 0 of the other events set up as of 1.11.0.Use sparkling spot on library floor to enter (marked "Filth!").Check the computer in the Beacon library to agree to terms and conditions (and set starting stats).Sarah - Manipulator (plus special shop to make her OP as all get out).Notable characters with adult themes kept barely under control: Yin - Mental progression from extreme pacifist to able fighter during her recruitment quest (with 5 possible final states).Deeda - Species-fluid.main has to choose angel (androgenous) or demon at startup, plus event (on the Patch-Vale bridge) to start freely changing once level 50 is reached (this is available as non-main as well, watch out for condition that makes her unavailable).Rona - Semblance allows her to change into a cat (tiger in battle).Jessica - Rapid growth from child to adult form (leave her starting semblance equipped and rest at inns 5 times).Blake, Kali, Sienna, Neon, Nora, Velvet, Wisp, Amethyst, Pyrrha, Candi, Coco, Emerald - Cat.Jaune (main only) - Many possibilities.same NPC as Ruby.Ruby (main only) - Many possibilities.find the "witch" in the alley next to the bar in Vale.v1.13.6x - Cats and Sharks and Sewers.v1.13.0 - Combatical Graphical Overhaul.ical.v1.12.9 - Failed to plan, or planned to fail?.Version number starting high, retained from year+ on game jolt. Don't believe me? Find or play as the OC "Olivia". Lots of things to do (see Walkthrough tab for list and where to find).Nearly every main-able character can be recruited to your party, plus quite a few that aren't available as mains.LOTS of playable main characters, both OC and canon (See Characters tab for ones that have TF). ![]() Based in RoosterTeeth's "RWBY: Chibi".as in, meaningless fun. Open world game using the same models, stats, etc as Team SARA. ![]() ![]() There are also genetics mods, clothing recolors, and build mode objects. Noodle Sorbets is a site dedicated to recolors of hairstyles. Gossip Girl is the queen of tattoos! She has many different styles and designs available on her tumblr. Beds that actually look like someone slept in them… not the perfect beds we’re used toįreckles and slept-in beds! 5. ![]() Modifications of existing clothing options from the game.Melly’s tumblr page is full of cute modifications of existing objects, like windows from the Moschino stuff pack. A motive decay mod that feels more true-to-lifeĪ Unique name for a unique collection of custom content 6.An invisible fill slot so you can place objects where you want them on surfaces.An air guitar that still builds guitar skill.This tumblr has some quirky, yet useful downloadable content. Also has lots of build mode items, like floors, walls, and houses/lotsīeautiful couches and recolorings are available on this site.Tons of buy mode items, including clutter, artwork, and object recolors.Hair and clothing styles are Maxis Match.This site has tutorials so you can learn to make your own content. Simsational Designs is another tumblr dedicated to providing free, downloadable custom content for The Sims 3 & 4. A few clothing sets for different styles, including a Coven Collectionĭownload a new version of Britechester for your Sims to enjoy.A totally revamped map of Britechester with new townies.Cute and stylish hairstyles for female Sims.There are over 300 pages of downloadable content for you to check out. LilSimseĪ tumbler by LilSimse, full of CC, particularly female hairstyles, that are all Maxis Match. It’s visual so you know what you’re downloadingĪ wide variety of CC options can be found on Pinterest.If you’ve never used Pinterest before, it’s an awesome site for finding content. You can find everything from clothes, to clutter, to hairstyles. PinterestĪ quick search on Pinterest for Sims CC brings up a lot of options, curated from different websites. Check out the top ten sites for killer custom content. CC can also liven up Sims storytelling videos and pictures. There’s plenty of CC for those who like to create sims and for those who like to build houses. ![]() ![]() Fear not! Custom Content, which has been around since the first game came out in 2000, is a great way to liven up your game, and bring some new variety to CAS mode and Build/Buy mode. Your enthusiasm and passion for my mods continue to motivate me.If you’re like me, you’ve logged hundreds or thousands of hours playing The Sims over the last twenty years.Īnd you’re bored. Thank you for being a part of this amazing community. I'm committed to improving my reliability and ensuring a more consistent modding experience for all of you. Please know that I'm working hard to get back on track and bring you the quality mods you deserve. I want to express my heartfelt gratitude for your ongoing support and understanding. However, I want you to know that I'm committed to taking care of my mental well-being and exploring ways to manage these potential conditions. It has been tough to navigate these challenges, and I haven't had the energy to seek professional therapy or a formal diagnosis. They bring me joy, but they also present unique challenges that sometimes leave me exhausted, on top of my 15 cats, I can't help but take care of any stray cats that come my way, which makes it extremely difficult to focus on anything.Īdditionally, based on symptoms that I have been dealing with, I suspect that I may be dealing with mental health issues like ADHD, bipolar disorder, and borderline personality disorder. I understand how frustrating it can be to have an inconsistent modding presence, and I'm genuinely sorry for any inconvenience or disappointment it may have caused.ĭespite my deep love for my 15 adorable cats, they have been quite the handful and have taken away a significant amount of my time and energy. Life has thrown me some unexpected curveballs, and it has been a challenge to find the balance and consistency I strive for. It's clear that unfortunately I haven't been consistent with my uploads and have been disappearing a lot in the past couple of years. I hope all of you are doing great and enjoying the mods. ![]() ![]() ![]() This makes it a quick 1-step morning routine. It can be worn under makeup, but it's also great on its own, and you don't necessarily need to moisturize underneath. If you're looking for a great mineral sunscreen that isn't matte, I recommend this one. I've also confirmed with the brand that it's reef-safe, which means it contains no nanoparticles. It doesn't leave a white cast and it's slightly tinted a light beige color. It is however very hydrating and has a natural, glowy finish. Even though it's a "moisturizer", I still use my regular moisturizer underneath. One of my favorite facial sunscreens is this Essential Glow Moisturizer by Paula's Choice. Paula's Choice Essential Glow Moisturizer SPF 30 (Face) You can always contact the brand directly if it's not obvious, like I have for some of the options below. Some brands will make the claim on the bottle if they are reef-safe, while others don't. Mineral sunscreens are also reef-safe as long as they don't use nano-particles. It's also a safe ingredient and should cause no adverse reactions or breakouts, if you're acne-prone. They're also all mineral sunscreens, meaning they contain zinc oxide, which blocks both UVB and UVA rays. ![]() The following sunscreens are all cruelty-free, and I've chosen options for both face or body. Cruelty-Free Sunscreen Picks: Mineral Sunscreen For Face And Body Click here to find out more and claim your May box. Every month, we bring you up to $195 worth of cruelty-free and vegan products for only $34.99 with our subscription box. ![]() ![]() ![]() In the Qarnot_blastn_example folder, follow these steps to set up a Python virtual environment. In this script, you need to enter your Qarnot Token linked to your account (you can find it here) to use our platform. Save the following script as run.py in your Qarnot_blastn_example folder. Now, let’s use Qarnot Python SDK to launch the distributed calculation. Tutorialīefore we start, you need to create a Qarnot account, we offer 15€ worth of computation on your subscription.įirst, in a Qarnot_blastn_example folder, create a folder named blastn_resources and save inside the following data which contains two local sequences. We will align a list of query DNA sequences against another list of reference DNA sequences. In this part we describe a simple example of using BLAST, and more particularly the tool blastn, on Qarnot, using the python SDK. The program compares nucleotide sequences to sequence databases and computes statistical significance.ĭepending on the sequencing data type, there are different specific tools, but in this article, we focus on the usage of blastn (which means the alignment of nucleotide sequences). BLASTīasic Local Alignment Search Tool (BLAST) is initially an online web-based tool allowing to find regions of similarity between biological sequences. Another representation of this kind of alignment is a sequence logo (see example below). In this way, the RNA sequence alignment can also be used to quantify the genes expression.įinally, the protein sequences alignment allows to visualize the conserved regions and motifs, giving a functional point of view from the most representative amino acids. The alignment is used with High-Throughput Sequencing (HTS) data, to match the query sequences with a known sequence, or de novo. The DNA sequence alignment allows to interpret the results as point mutations, insertions or deletions, such as Single Nucleotide Polymorphism (SNP) or Single Nucleotide Variant (SNV). A score is attached to each alignment result, based on the similarity and sequence complexity. The final output is globally a human-interpretable text file, showing the mismatches of gaps between the queries and the reference sequence(s). The second one is called the reference, or database, which is the set of sequences that get compared with the query. The first dataset contains the query, which means the sequence(s) we need to analyse. In common cases, we have two datasets in input, containing both one or more sequences. This is why the DNA, RNA or protein sequences analysis became a key challenge in biology, and a first evident problem held in comparing huge amounts of sequencing data.Ī sequence alignment is a bioinformatics method allowing to rearrange and compare two sequences, mostly of the same kind (DNA, RNA or protein). This information is also defined as the genetic code, and works as an orchestrator for the other system levels, from the proteins to cells, tissues and organs. Sequence alignment DefinitionĭeoxyriboNucleic Acid, also known as DNA, is the basic information of any living organism. This blog post introduces the concept of sequence alignment, the BLAST algorithm, and an example of how to use it on Qarnot. ![]() |